Towards Polarizing Correlative Light-Electron Microscopy (PCLEM).

microscopy and analysis Correlative Light and Electron Microscopy

Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function

Many core paradigms of contemporary neuroscience are based on information obtained by electron or light microscopy. Intriguingly, these two imaging techniques are often viewed as complementary, yet separate entities. Recent technological advancements in microscopy techniques, labeling tools, and fixation or preparation procedures have fueled the development of a series of hybrid approaches that...

Multi-color correlative light and electron microscopy using nanoparticle cathodoluminescence

Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images, and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: multi-color imaging of stable ...

Correlative light- and electron microscopy with chemical tags.

Correlative microscopy incorporates the specificity of fluorescent protein labeling into high-resolution electron micrographs. Several approaches exist for correlative microscopy, most of which have used the green fluorescent protein (GFP) as the label for light microscopy. Here we use chemical tagging and synthetic fluorophores instead, in order to achieve protein-specific labeling, and to per...

Correlative Light and Electron Microscopy – on the Way from 2D Towards 3D

The next step will be to address correlative 3D applications. To achieve this, it is necessary to exactly define volumes of interest (VOI) in the data of the first microscope. Further, the precise relocation of the identical VOI in the second microscope is essential as well as the registration of the 3D object in all spatial directions. However, the correlation of 3-dimensional data from differ...